The cyanobacteria spirulina platensis is an excellent source of c. Cphycocyanin extraction from spirulina platensis oven. Processingindependent extracellular production of high. Spirulina platensis is one of the popular species that containing cphycocyanin. Among many of spirulina bioactive components, blue. Sivasankari, naganandhini and david ravindran department of biology, gandhigram rural. Phycocyanin extraction from cyanobacteria spirulina platensis was optimized using factorial design and response surface techniques. B four photobioreactors 50 l tanks with initial volumes of 20 l of s. These data suggest that the phycocyanin isolated using the highpressure process and hexane separation method, compared to two other samples, was more stable because the low. Phycocyanin is a pigmentprotein complex from the lightharvesting phycobiliprotein family, along with allophycocyanin and phycoerythrin. Medical application of spirulina platensis derived cphycocyanin qianliu, 1 yinghonghuang, 1 ronghuazhang, 1 tiangecai, 2 andyucai 1,3. Along with the development of marine biological pharmaceutical research, higheffective and lowtoxic drugs and functional foods isolated from marine organisms have become a new field of pharmacy and. Cphycocyanin extraction from spirulina platensis wet. The interaction of znii ions with cyanobacteria spirulina platensis basic protein cphycocyanin cpc is studied by fluorescence spectroscopy.
In this paper we describe the extraction and purification of cphycocyanin from the cyanobacterium spirulina platensis. Study of phycocyanin production from spirulina platensis braz. Stable isolation of phycocyanin from spirulina platensis. Optimization of phycocyanin extraction from spirulina platensis. In the present study, a simple and efficient method to extract cphycocyanin from spirulina platensis wet biomass is reported. Spirulina has a documented history of use as a food for more than years, and has been in production as a dietary supplement for 40 years. A simple method for efficient extraction and separation of. Optimization of phycocyanin extraction from microalgae spirulina.
Extraction and purification of cphycocyanin from dry. The commercial production of spirulina has long been implemented in ponds, but photobioreactors and other innovating culture techniques tend to develop. Comparison of different extraction methods for yield of phycocyanin comparison was made among different procedures for phycocyanin extraction with freshly harvested biomass and the data are. Production of antioxidant cphycocyanin using extraction. Among many of spirulina bioactive components, blue protein cphycocyanin and its. Cphycocyanin has been used in many applications such as antioxidant, antiinflammatory, and natural blue colorant. Pc extraction with food grade purity from wet spirulina platensis biomass is proposed. Antioxidant activity optimisation of spirulina platensis c. Sternvolmer quenching constant value for zniicpc is. Extraction and purification of cphycocyanin from spirulina. Phycocyanin is a potent antioxidant and possesses significant immune enhancing and. In vitro antioxidant activities of cphycocyanin extracts obtained from spirulina platensis by freezethaw, microwaveassisted extraction mae and ultrasoundassisted extraction uae methods were.
The experimental data on the study of the antibacterial activity of purified phycocyanin, a proteinbound pigment isolated from bluegreen alga, spirulina platensis nordstedt geitler, oscillatoriaceae are. Extraction and purification of cphycocyanin from spirulina platensis ccc540 article pdf available in indian journal of plant physiology 192. Spirulina phycobiliproteins as food components and. Comparison of different extraction methods for phycocyanin. Extraction of phycocyanin from spirulina plantesis using. The aim of this work was to investigate the production of phycocyanin by spirulina platensis under different spectra of light. The effects of temperature and biomasssolvent ratio on phycocyanin. Phycocyanin was extracted from the wet biomass of spirulina by using the below methods. Quantitative analysis of cphycocyanin from spirulina. A simple method for extracting cphycocyanin from spirulina.
Pdf cphycocyanin extraction from spirulina platensis. Cphycocyanin cpc is a natural blue colored protein of spirulina platensis. Cphycocyanin cpc was extracted from fresh spirulina platensis. In order to extract phycocyanin from spirulina platensis cells, selection of ultrasonic frequency was important. Phycocyanin is a major light harvesting pigment of cyanobacteria, it has gained importance in many biotechnological applications in food sciences, therapy, immunodiagnostics, cosmetics and. Review article medical application of spirulina platensis. Purified cphycocyanin from spirulina platensis nordstedt. In the present study, a simple and efficient method to extract cphycocyanin from spirulina platensis wet biomass is.
Pure freeze dried blue phycocyanin spirulina extract powder. Production of cphycocyanin and its potential applications. No fillers, binders, or flow agents have been added. Spirulina platensis, arthrospira platensis ingredients. Cphycocyanin cpc, a phycobiliprotein, is one of the key pigments of spirulina, a microalgae used in many countries as a.
Cell pretreatment with ethylenediaminetetraacetic acid for. Cphycocyanin cpc is a phycobiliprotein found in blue green algae, such as spirulina platensis, is often used as a dietary nutritional supplement and exhibit a variety of pharmacological. Phyocyanin extraction from microalgae spirulina platensis. The extraction efficiency of phycocyanin and the influence of different process parameters e. Phycocyanin extraction is carried out from wet or dry. The optimized concentration and purity of spirulina. Astonishingly rich in nutrients, spirulina is one of the most popular and well researched functional foods in the multibillion dollar global food supplement market. Phycocyanin is an important commercially available blue food colorant. A number of extracting methods have been studied to isolate this blue pigment from spirulina. The yield of phycocyanin in crude extract was depended on ultrasonic. An extensive range of pigments including phycobiliproteins are present in algae. The cells were pretreated with ethylenediaminetetraacetic acid and subsequent c.
This paper presents the evaluation of some important parameters for the purification of phycocyanin using ion exchange chromatography. Phycocyanin extraction in spirulina produced using. Research article phycocyanin extraction from spirulina platensis. Cphycocyanin is a natural blue dye used in food and pharmaceutical industry. Phycocyanin is a watersoluble blue pigment found only in spirulina and other species of bluegreen microalgae.
Recent developments in production and biotechnological. Pdf extraction and purification of cphycocyanin from. Cphycocyanin extraction from spirulina platensis wet biomass. Efficacy of different extraction methods of phycocyanin. Separation of phycocyanin from spirulina platensis using. In vitro antioxidant activities of cphycocyanin extracts obtained from spirulina platensis by freezethaw, microwaveassisted extraction mae. Extraction and separation of phycocyanin from spirulina. Comparison of different extraction methods for phycocyanin extraction and yield from spirulina platensis s. Xifeng zhang 1, fenqin zhang 1, guanghong luo 2, shenghui yang 2 and danxia. The dependent variables evaluated were the amount of phycocyanin. Spirulina phycobiliproteins as food components and complements.
Herein we report an optimization study of various phycocyanin extraction methods from spirulina platensis cyanobacterium biomass. Spirulina and other bluegreen algae contain cphycocyanin, which acts as an accessory pigment when light energy is captured and transferred to chlorophyll a. Phycocyanin extraction in spirulina produced using agricultural. Conformational studies of phycocyanin from spirulina platensis. Spirulina is used as a high quality protein mainly for phycocyanin eriksen 2008, which is a important cyanobacterial accessory pigment having a number of industrial applications. Study of phycocyanin production from spirulina platensis. Extraction and purification of cphycocyanin from spirulina platensis employing aqueous two phase systems. Phycocyanin powder, broken cell blue spirulina powder. Purification of cphycocyanin from spirulina platensis by single. It contains phycocyanin, a blue pigment, which is known as a nutritious food.
A method for stably purifying a functional dye, phycocyanin from spirulina platensis was developed by a hexane extraction process combined with high pressure. Medical application of spirulina platensis derived c. Several methods based on chemical, physical, and enzymatic processes have been reported for the extraction of cpc. Extraction and separation of phycocyanin from spirulina using aqueous twophase systems of ionic liquid and salt.
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